{"id":646,"date":"2026-05-15T21:55:06","date_gmt":"2026-05-16T00:55:06","guid":{"rendered":"https:\/\/departamentos.ufrrj.br\/icbs-dcfis\/?p=646"},"modified":"2026-05-15T21:55:07","modified_gmt":"2026-05-16T00:55:07","slug":"laboratorio-de-investigacao-cardiometabolica","status":"publish","type":"post","link":"https:\/\/departamentos.ufrrj.br\/icbs-dcfis\/2026\/05\/15\/laboratorio-de-investigacao-cardiometabolica\/","title":{"rendered":"Laborat\u00f3rio de Investiga\u00e7\u00e3o Cardiometab\u00f3lica"},"content":{"rendered":"\n
    \n
  1. Laborat\u00f3rio de Investiga\u00e7\u00e3o Cardiometab\u00f3lica<\/strong><\/li>\n<\/ol>\n\n\n\n

    Respons\u00e1vel: Norma Aparecida Almeida Figueiredo de Oliveira<\/p>\n\n\n\n

    Localiza\u00e7\u00e3o: Sala 3E do PQ<\/p>\n\n\n\n

    Medida: 31,00 m2<\/sup><\/p>\n\n\n\n

    1. Descri\u00e7\u00e3o das atividades do laborat\u00f3rio<\/p>\n\n\n\n

    O laborat\u00f3rio surgiu oficialmente em 2022 atrav\u00e9s da Portaria n\u00famero 5222\/2022 sob a coordena\u00e7\u00e3o da Professora Norma Aparecida Almeida Figueiredo de Oliveira. O LIC \u00e9 uma unidade acad\u00eamica especializada vinculada ao Departamento de Ci\u00eancias Fisiol\u00f3gicas do Instituto de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade. No laborat\u00f3rio s\u00e3o realizados projetos em pesquisa b\u00e1sica nas \u00e1reas de fisiologia card\u00edaca e end\u00f3crina, que integram o ensino de gradua\u00e7\u00e3o e p\u00f3s-gradua\u00e7\u00e3o, com \u00eanfase em aspectos moleculares. No laborat\u00f3rio \u00e9 realizado a extra\u00e7\u00e3o de prote\u00ednas e realiza\u00e7\u00e3o da t\u00e9cnica de western blot para investiga\u00e7\u00e3o do padr\u00e3o de express\u00e3o proteica em tecidos-alvos; a extra\u00e7\u00e3o de mRNA e realiza\u00e7\u00e3o de RT-PCR e q-PCR para caracteriza\u00e7\u00e3o do padr\u00e3o de express\u00e3o g\u00eanica , extra\u00e7\u00e3o de lip\u00eddeos para quantifica\u00e7\u00e3o de lip\u00eddeos neutros em tecidos-alvos atrav\u00e9s da t\u00e9cnica de cromatografia em camada delgada monodimensional ou Thin Layer Chromatography (TLC), ensaios enzim\u00e1ticos baseados em rea\u00e7\u00f5es colorim\u00e9tricas e testes de toler\u00e2ncia a glicose. As linhas de pesquisa em andamento investigam os efeitos de programa\u00e7\u00e3o materna atrav\u00e9s de dietas obesog\u00eanicas sobre a fun\u00e7\u00e3o card\u00edaca e o efeito do diabetes tipo II sobre o cora\u00e7\u00e3o. Al\u00e9m disso s\u00e3o realizadas a\u00e7\u00f5es de extens\u00e3o em que s\u00e3o abordados temas sobre educa\u00e7\u00e3o alimentar.<\/p>\n\n\n\n

    2. Complemento<\/p>\n\n\n\n

    Descri\u00e7\u00e3o sucinta dos equipamentos<\/p>\n\n\n\n

    – Termociclador: Termociclador bloco com 96 po\u00e7os, marca Veriti (1)<\/p>\n\n\n\n

    – Aparatos para eletroforese em gel vertical e transfer\u00eancia de prote\u00ednas: Mini Protean Tetra Cell e Mini Trans-Blot Cell,marca BIO-RAD (1)<\/p>\n\n\n\n

    – Forno para hibridiza\u00e7\u00e3o: HB 1000 Forno para hibridiza\u00e7\u00e3o, marca Hoefer (1)<\/p>\n\n\n\n

    – Sistema de purifica\u00e7\u00e3o de \u00e1gua ultra pura Tipo 1: DI RECT-Q 3 UV SMART, PRODUZ AGUA TIPO III VAZAO DE 3 LPH E TIPO I VAZAO DE 30 LPH, marca Merck Millipore (1)<\/p>\n\n\n\n

    – Centr\u00edfuga refrigerada para microtubos: CENTR\u00cdFUGA REFRIGERADA DE BANCADA DIGITAL – VEL. MAX. 6.000 RPM P\/ ROTOR DE \u00c2NGULO FIXO, marca Splabor (1)<\/p>\n\n\n\n

    – Balan\u00e7a anal\u00edtica eletr\u00f4nica: HR200X, marca AND (1)<\/p>\n\n\n\n

    – Espectrofot\u00f4metro: NanoDrop ND-2000, marca NanoDrop (1)<\/p>\n\n\n\n

    – Placa de agita\u00e7\u00e3o com aquecimento (1)<\/p>\n\n\n\n

    – Homogeinizador de tecido, tipo turrax (1)<\/p>\n\n\n\n

    – Capela com exautor (1)<\/p>\n\n\n\n

    – Geladeira (1)<\/p>\n\n\n\n

    – Freezer (1)<\/p>\n\n\n\n

    3. Quantidade de equipamentos<\/p>\n\n\n\n

    12<\/p>\n\n\n\n

    4. *Palavras-chave*: Fisiologia card\u00edaca, fisiologia end\u00f3crina, programa\u00e7\u00e3o metab\u00f3lica.<\/p>\n\n\n\n

    5. *Capacidade de alunos*<\/p>\n\n\n\n

    Seis<\/p>\n","protected":false},"excerpt":{"rendered":"

    Respons\u00e1vel: Norma Aparecida Almeida Figueiredo de Oliveira Localiza\u00e7\u00e3o: Sala 3E do PQ Medida: 31,00 m2 1. Descri\u00e7\u00e3o das atividades do laborat\u00f3rio O […]<\/p>\n","protected":false},"author":17,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-646","post","type-post","status-publish","format-standard","hentry","category-sem-categoria"],"acf":[],"yoast_head":"\nLaborat\u00f3rio de Investiga\u00e7\u00e3o Cardiometab\u00f3lica - Departamento de Ci\u00eancias Fisiol\u00f3gicas<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/departamentos.ufrrj.br\/icbs-dcfis\/2026\/05\/15\/laboratorio-de-investigacao-cardiometabolica\/\" \/>\n<meta property=\"og:locale\" content=\"pt_BR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Laborat\u00f3rio de Investiga\u00e7\u00e3o Cardiometab\u00f3lica - Departamento de Ci\u00eancias Fisiol\u00f3gicas\" \/>\n<meta property=\"og:description\" content=\"Respons\u00e1vel: Norma Aparecida Almeida Figueiredo de Oliveira Localiza\u00e7\u00e3o: Sala 3E do PQ Medida: 31,00 m2 1. 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